Research

AF-8 does not contain: Gluten, wheat, added preservatives, artificial colouring or any genetically modified material and is suitable for vegetarians.

NITRIC OXIDE (NO) is one of the simplest molecules in biology, comprised of just two atoms-one atom of nitrogen (N) and one of oxygen (O). Though nitric oxide’s structure is simple, nitric oxide is now regarded as the most significant molecule in the body, one that is crucial to a person’s well-being.

Nitric oxide is a powerful signalling molecule present in the cardiovascular and nervous systems as well as throughout the rest of the body, influencing the functioning of virtually every bodily organ, including the lungs, liver, kidneys, stomach, genitals, and, of course, the heart. Among the many vital duties nitric oxide performs is its role as a vasodilator, meaning that it helps control blood flow to every part of the body. Nitric oxide relaxes and enlarges the blood vessels, ensuring that blood can efficiently nourish the heart, helping to support healthy blood pressure levels.

Nitric oxide also works to prevent the formation of blood clots, which can be a trigger for strokes and heart attacks, whilst slowing the accumulation of atherosclerotic plaque in the blood vessels along with supporting the immune system, combating toxins and providing a strong internal defence system. Nitric oxide therefore plays a vital role in the health of your arteries, and thus your heart and therefore your quality of general health.

The manufacture of nitric oxide takes place mainly in the endothelium-which is the layer of cells lining the interior surface of the blood vessels. Heart disease, being overweight, lack of exercise, smoking, high cholesterol, high blood pressure and high levels of homocysteine all, amongst other issues, cause damage to the endothelium. And a damaged endothelium is clearly not conducive to the production of nitric oxide, which results in more damage and an increasingly dangerous downward spiral of declining health.

A damaged endothelium is not the only issue we need to address. Whilst L-Arginine is an amino acid that can produce nitric oxide in your system, unfortunately, the amounts that we consume from our food naturally are insufficient to make the difference in nitric oxide production that is required to stimulate health benefits. Supplementation is the best way to ensure a healthy endothelium resulting in nitric oxide production at its best.

CLINICAL EVIDENCE FOR INGREDIENTS OF AF-8

L-Arginine

5.25g per serving

L-Citrulline

1g per serving

Vitamin C

0.45g per serving
(525% NVR)

Beetroot
Extract

0.21g per serving

Grape Seed
Extract

0.08g per serving

Resveratrol
Extract

0.0315g per serving

Vitamin K2

180mcg per serving
(240% NRV)

Vitamin D3

1000iu per serving
(500% NRV)

SYNERGISTIC RELATIONSHIPS
Citruline and Arginine

Citrulline and arginine scientific studies

A study in humans showed the citrulline supplementation’s “time release” effect on arginine production. In this study an oral dose of 1 gram of citrulline resulted in a 227% peak increase in plasma arginine levels after 4 hours, compared with a 90% peak increase with the same dose of arginine.

Oral administration of citrulline alongside arginine appears to be considerably more efficient at raising plasma levels of arginine over the long term than arginine by itself.

Reduces blood pressure

L-Arginine and L-Citrulline supplementation combination has shown to reduce blood pressure in 17 young (average age 21.6 years) men with normal blood pressure after they were submitted to a cold pressor test (CPT). (A cold pressor test is a cardiovascular test done by having the subject immerse his hand into a bucket of ice water for one minute. Blood pressure and heart rate are then evaluated). The men were randomly assigned to four weeks of oral citrulline and arginine supplementation or placebo in a crossover design. Blood pressure was measured after the blood pressor test.

The results showed that compared to placebo, oral citrulline and arginine decreased brachial systolic blood pressure (-6 +/- 11 mm Hg), aortic systolic blood pressure (-4 +/- 10 mm Hg), and aortic pulse pressure (-3 +/- 6 mm Hg) during CPT.

Even more importantly, new studies are showing that supplemental citrulline also assists in nitric oxide production by boosting blood levels of arginine.

SYNERGISTIC RELATIONSHIPS
Vitamin C and Arginine

A study published in the journal of sports and science medicine has found that vitamin C combined with arginine increases NO levels more than just arginine alone. Supposedly, vitamin C destroys free radicals that break down nitric oxide.

Objective

According to the literature L- Arginine supplementation improves physical performance by decreasing fatigue due to nitric oxide (NO) vasodilatation effect. This investigation sought to assess the effect of L-Arginine supplementation on strength and body composition of young soccer players during an 8-week weight training protocol.

Methods

20 soccer players, age between 17 and 19 years old (mean 17.65 ± 0.8 yrs. were supplemented either with 3 g of L-arginine plus 1 g of vitamin C (group ARG) or just with 1 g of vitamin C (group CON). They underwent eight weeks of weight training (3 times/ week). Statistical analyses used were ANOVA and “t” test.

Results

Group ARG showed a significant increase in body-weight (66.4 ± 6.1; 67.84 ± 6.8 kg), lean body mass (60.38 ± 6.05; 62.07± 5.9 kg) and muscular strength of both legs, right(R) and left(L) (Extension R 184.8 ± 17.4 to 195.8 ± 16.3; L 191.1 ± 18.4 to 199.1 ± 19.1), and a decrease in body fat (6.02 ± 0.6 – 5.77 ± 0.59 Kg) and %body fat (9.45 ± 0.8 to 8.66 ± 0.77) (p<0.05). There was no significant change in CON group.

CLINICAL EVIDENCE FOR MAINTAINING REDUCED CHOLESTEROL WHEN TAKING AF-8

Female patient (80 years) presented with very high cholesterol (over 6.5) and was put on statins by her Doctor-which successfully reduced her cholesterol. Patient did not like taking the statins as she experienced very unpleasant side effects and so told her Doctor she was going to stop taking them. Her cholesterol went back up, so her Doctor insisted that she go back on statins again-which she did and her cholesterol came down again to 5.2. Patient asked her Doctor if she could stop the statins for 3 months and take AF-8. The Doctor agreed on the proviso he took blood tests so he could monitor and check. He took blood tests (Results-A) at the start of the 3 months, but cautioned patient that he wanted to retest her after the 3 months and if her cholesterol had gone back up again she would need to start taking statins again.

Results A

Before

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Results B

After

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*Always read the label. Use only as directed. Vitamin Supplements should not replace a balanced diet. If symptoms persist consult your healthcare professional.